This report presents results of independent laboratory tests conducted on the effectiveness of ozone against five stains of microorganisms. The test subjects were chosen because they represent common problem in our modern living. Three bacteria were chosen. One especially for its hardiness and resistance to chemicals. One yeast ndida, and one mold , Aspergillus. The tests were conducted using one of our air purifiers which produced About. 05 ppm of ozone. This is the limit below which the FDA does not recognize ozone production. If we had chosen a lower level the results would have been the same with perhaps the 1\2 hour readings not quiteas dramatic. From the data and the graphical representations it can be seen that dramatic, quick and effective destruction of all of the test subjects was accomplished. Note the 1\2 hour results. Note also that after some time, if total destruction is not achieved, the orgamisms are able to re-establish themselves. This shows the necessity of continuous use of the product. SUMMARY: From the results of the tests, after four hours exposure to ozone, Present between 0.049 and 0.064 parts per million generated by the air purifier, number of on five tested microorganisms were significantly reduced on the test surfaces when compared to control surfaces exposed to normal room air. METHODOLOGY: A containment chamber constructed from a polyethylene glove bag was used for the ozone exposure. Once the chamber atmosphere reached a relatively stable 0.05 parts per million ozone concentration, stainless steel Penicillin ring carriers, were coated with 48 hour culture suspensions and dried for 30 minutes at 35 c. Test carriers were exposed to ozone for 0,1/2,1,2, and 4 hours intervals. Control carriers were exposed to normal room air for 4 hours. After their respective exposures, each carrier was immersed in a tube of buffered ozone-neutralizing solution. These tubes were held in an ice bath in the chamber. The ice bath also served to maintain a high humidity. At the end of the test period, the chamber was opened, and the control carriers’ tubes added to the ice bath until quantification. Quantification was performed by serial dilution and plating on Lactose agar for the bacteria; on Mycological agar for the fungi [mold]. Adapted from AOAC Method 4.010.
|